|
|
|
Registros recuperados: 26 | |
|
|
Paillard, Christine; Leroux, Frederique; Borrego, Juan. |
The main microbial diseases affecting marine cultured bivalves have been revised on the basis of the etiologic agents, pathogenesis and pathogenicity. Several recent bivalve-interaction models have been studied, including Pecten larvae-Vibrio pectinicida, brown ring disease, juvenile oyster disease, Pacific oyster nocardiosis and summer mortalities of oysters. In addition, the taxonomy and phylogeny of new potential bivalve pathogens and their virulence factors have been established. Facing the difficulty of identifying bacterial strains associated with molluscan diseases (mainly vibriosis), polyphasic approaches have been developed to correlate the phenotype and genotype of potential pathogens. By evaluating likely virulence mechanisms, developing... |
Tipo: Text |
Palavras-chave: Diagnostic tools; Virulence factors; Pathogenicity; Pathogenesis; Etiology; Bivalve mollusc; Vibriosis. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-407.pdf |
| |
|
|
Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene. |
Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical... |
Tipo: Text |
Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf |
| |
|
|
Goyard, Emmanuel; Goarant, Cyrille; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Galinie, C; Peignon, Jean-marie; Pham, Dominique; Vourey, Elodie; Harache, Yves; Patrois, Jacques. |
Two populations of the Latin American shrimp Penaeus (Litopenaeus)stylirostris domesticated in Hawaii and in New Caledonia were previously shown to be genetically differentiated and proven highly inbred. In New Caledonia, where different Vibriosis affect shrimp production and antibiotic use is banned in growing ponds, the Hawaiian population was introduced to increase the allelic variability available for local shrimp farmers and start a genetic improvement program. Growth and survival rates of the two pure populations and the two-way F-1-hybrids obtained by breeding Hawaiian animals with New Caledonian animals were assessed in several simple experiments (earthen ponds, floating cages and experimental infection challenges) during two years on two... |
Tipo: Text |
Palavras-chave: Vibriosis; Survival; Growth; Penaeus Litopenaeus stylirostris; Shrimp; Cross breeding; Genetic improvement. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4316.pdf |
| |
|
|
Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly. |
New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government... |
Tipo: Text |
Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf |
| |
|
|
Le Roux, Frederique. |
Global changes linked to increases in temperature and ocean acidification, but also to more direct anthropogenic influences such as aquaculture, have caused a worldwide increase in the reports of Vibrio-associated illnesses affecting humans and also animals such as shrimp and molluscs. Investigation of the emergence of Vibrio pathogenesis events requires the analysis of microbial evolution at the gene, genome and population levels, in order to identify genomic modifications linked to increased virulence, resistance and/or prevalence, or to recent host shift. From a more applied point of view, the elucidation of virulence mechanisms is a prerequisite to devising prophylactic methods to fight infectious agents. In comparison with human pathogens, fairly... |
Tipo: Text |
Palavras-chave: Écologie; Émergence; Évolution; Invertébrés marins; Vibriose; Virulence; Ecology; Emergence; Evolution; Marine invertebrate; Vibriosis; Virulence. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00344/45560/46722.pdf |
| |
|
|
Lemonnier, Hugues; Lantoine, Francois; Courties, Claude; Guillebault, Delphine; Nezan, Elisabeth; Chomerat, Nicolas; Escoubeyrou, Karine; Galinie, Christian; Blockmans, Bernard; Laugier, Thierry. |
Tropical shrimp aquaculture systems in New Caledonia regularly face major crises resulting from outbreaks of Vibrio infections. Ponds are highly dynamic and challenging environments and display a wide range of trophic conditions. In farms affected by vibriosis, phytoplankton biomass and composition are highly variable. These conditions may promote the development of harmful algae increasing shrimp susceptibility to bacterial infections. Phytoplankton compartment before and during mortality outbreaks was monitored at a shrimp farm that has been regularly and highly impacted by these diseases. Combining information from flow cytometry, microscopy, pigment and phylogenetic analysis, the presence of Picocyanobacteria, Prasinophyceae and Diatomophyceae were... |
Tipo: Text |
Palavras-chave: Tropical aquaculture pond; Litopenaeus stylirostris; Vibriosis; Eutrophication; Phytoplankton; Harmful algae. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00343/45411/44986.pdf |
| |
|
|
Maes, P; Paillard, C. |
The symptom which characterizes the brown ring disease (BRD) in Manilla clam Ruditapes philippinarum has been observed in other Bivalvia species (Veneridae and Pectinidae). Vibrio P1, responsible of the BRD in R. philippinarum , has been detected in other Veneridae which were carrying the symptom. In mussel and oysters which are cultivated in Europe, the symptom has never been reported and Vibrio P1 has not been detected. Experimental contaminations of bivalves of commercial interest with the pathogen showed a clear Vibrio P1 sensitivity of European clam R. decussatus ; this latter seems however to be less sensitive than the Manilla clam. |
Tipo: Text |
Palavras-chave: Ruditapes philippinarum; Bivalvia; Diseases; Marine molluscs; Pathology; Vibriosis. |
Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-926.pdf |
| |
|
|
Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet. |
in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first experiment, the shrimps were found to be healthy over the trial period; no mortalities and non-detectable signs of infection were recorded. These resulted in high final survival rates (above 90% in both treatments). On the other hand, during the second trial, carried out at... |
Tipo: Text |
Palavras-chave: Vibriosis; Antioxidant defences; Oxidative stress; Probiotic; Shrimps. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6780.pdf |
| |
|
| |
|
| |
|
| |
|
| |
|
|
Elsersy, N.A.; Abo-Ella, G.M.; El-Shenawy, M.; Abdel-Mawla, E.. |
El-Max fish farm is one of the most important natural fish farm in Alexandria which is suffering from die-off of large amounts of fish. Facing this problem we must deal with the estimation of the quality of the farm raring unit environment (ponds). Analysis of samples using the Bray - Curtis similarity coefficient test yielded that, the input and El-Khandac were similar at (97%), while the eight ponds were classified at different level of similarity as follows: pond1 (B1) and pond7 (B7) were similar at (86%), B2 and B3 were similar at (90%), B14, B10 and B9 were similar at (93%), where as B11 is singly separated at (83%). Moreover, results indicate that distribution of the different bacterial groups was affected with one or more chemical parameters such as... |
Tipo: Journal Contribution |
Palavras-chave: Vibriosis; Fish farm; E. coli; Streptococcus faecalis Microbiological analysis Microbiology Fish farms Microbiological analysis Microbiology http://aims.fao.org/aos/agrovoc/c_25302 http://aims.fao.org/aos/agrovoc/c_4800. |
Ano: 2005 |
URL: http://hdl.handle.net/1834/1262 |
| |
|
|
Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Saulnier, Denis; Leroux, Frederique. |
A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp... |
Tipo: Text |
Palavras-chave: Virulence; Epidemiology; Typing; MLST; AP PCR; Mariculture; Vibriosis. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1913.pdf |
| |
|
|
Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique. |
Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V... |
Tipo: Text |
Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf |
| |
|
| |
|
|
Polsenaere, Pierre; Soletchnik, Patrick; Le Moine, Olivier; Gohin, Francis; Robert, Stephane; Pepin, Jean-francois; Stanisiere, Jean-yves; Dumas, Franck; Bechemin, Christian; Goulletquer, Philippe. |
In the context of global change, increasing mariculture production has raised particular concerns regarding its environmental impact and sustainability. Molluscs and particularly blue mussel account for a significant part of this total production. Although blue mussels are considered as pretty resilient to environmental disturbances, we report in this study an unprecedented mussel mortality event that occurred during the winter 2014 in the Breton Sound. 9000 metric tonnes of mussels were lost and mortality rates up to 100% were recorded at some farming areas. Through a coupling approach, the present work aimed to better understand the potential environmental drivers associated to those mortalities. Firstly we analysed long-term in situ and satellite data... |
Tipo: Text |
Palavras-chave: Mytilus edulis mortalities; Climate change; Breton sound; Long-term monitoring; Hydrodynamic modelling; Vibriosis. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00376/48708/49083.pdf |
| |
|
| |
|
|
Castex, Mathieu; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Wabete, Nelly; Nicolas, Jean-louis; Schmidely, P; Mariojouls, C. |
We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases... |
Tipo: Text |
Palavras-chave: Vibriosis; Floating cages; Litopenaeus stylirostris; Pediococcus acidilactici; Probiotic. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4079.pdf |
| |
|
|
Goarant, Cyrille; Merien, F. |
Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular... |
Tipo: Text |
Palavras-chave: Vibriosis; Vibrio; Real time PCR; Quantification; Mariculture; Extraction techniques. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1903.pdf |
| |
Registros recuperados: 26 | |
|
|
|